Utilisateur:MagaliN/Gammaproteobacteria rimP leader

The γ-proteobacterial rimP-leader is a putative attenuator element identified by bioinformatics^[1] within bacteria of the γ-proteobacterial phylum. It is located upstream of the rimP-nusA-infB operon encoding RimP, a protein shown to be involved in the 30S ribosomal subunit maturation^[2], NusA, a transcriptional factor controling termination, and the translation initiation factor IF-2 respectively. The rimP-leader presents a Rho-independent terminator at the 3' end, corresponding to a highly conserved GGGc(...)gCCC motif. This RNA is presumed to operate as a non-coding leader. Its mechanism remains unknown, but it is tempting to speculate a regulatory involvement of the NusA protein, which expression has been shown to lower the operon expression^[3]^[4], and which is already involved in the attenuation of the Trp, His and S10 operons^[5].

References[modifier | modifier le code]

↑ Naville M, Gautheret D, « The dark side of attenuation: large scale detection of functional 5' terminators in bacteria », Genome Biol. accepted,‎ 2010
↑ Nord S, Bylund GO, Lövgren JM, Wikström PM, « The RimP protein is important for maturation of the 30S ribosomal subunit », J Mol Biol, vol. 386, n^o 3,‎ 2009, p. 742-753
↑ Plumbridge JA, Dondon J, Nakamura Y, Grunberg-Manago M, « Effect of NusA protein on expression of the nusA,infB operon in E. coli », Nucleic Acids Res, vol. 13, n^o 9,‎ 1985, p. 3371-3388
↑ Nakamura Y, Plumbridge J, Dondon J, Grunberg-Manago M, « Evidence for autoregulation of the nusA-infB operon of Escherichia coli », Gene, vol. 36, n^os 1-2,‎ 1985, p. 189-193
↑ Worbs M, Bourenkov GP, Bartunik HD, Huber R, Wahl MC, « An extended RNA binding surface through arrayed S1 and KH domains in transcription factor NusA », Mol Cell, vol. 7, n^o 6,‎ 2001, p. 1177-1189

Gammaproteo_rimP-leader alignment [1]

[1] Naville M, Gautheret D, « The dark side of attenuation: large scale detection of functional 5' terminators in bacteria », Genome Biol. accepted,‎ 2010

[2] Nord S, Bylund GO, Lövgren JM, Wikström PM, « The RimP protein is important for maturation of the 30S ribosomal subunit », J Mol Biol, vol. 386, n^o 3,‎ 2009, p. 742-753

[3] Plumbridge JA, Dondon J, Nakamura Y, Grunberg-Manago M, « Effect of NusA protein on expression of the nusA,infB operon in E. coli », Nucleic Acids Res, vol. 13, n^o 9,‎ 1985, p. 3371-3388

[4] Nakamura Y, Plumbridge J, Dondon J, Grunberg-Manago M, « Evidence for autoregulation of the nusA-infB operon of Escherichia coli », Gene, vol. 36, n^os 1-2,‎ 1985, p. 189-193

[5] Worbs M, Bourenkov GP, Bartunik HD, Huber R, Wahl MC, « An extended RNA binding surface through arrayed S1 and KH domains in transcription factor NusA », Mol Cell, vol. 7, n^o 6,‎ 2001, p. 1177-1189

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