Utilisateur:MagaliN/Gammaproteobacteria rimP leader
The γ-proteobacterial rimP-leader is a putative attenuator element identified by bioinformatics[1] within bacteria of the γ-proteobacterial phylum. It is located upstream of the rimP-nusA-infB operon encoding RimP, a protein shown to be involved in the 30S ribosomal subunit maturation[2], NusA, a transcriptional factor controling termination, and the translation initiation factor IF-2 respectively. The rimP-leader presents a Rho-independent terminator at the 3' end, corresponding to a highly conserved GGGc(...)gCCC motif. This RNA is presumed to operate as a non-coding leader. Its mechanism remains unknown, but it is tempting to speculate a regulatory involvement of the NusA protein, which expression has been shown to lower the operon expression[3][4], and which is already involved in the attenuation of the Trp, His and S10 operons[5].
References
[modifier | modifier le code]- Nord S, Bylund GO, Lövgren JM, Wikström PM, « The RimP protein is important for maturation of the 30S ribosomal subunit », J Mol Biol, vol. 386, no 3, , p. 742-753
- Plumbridge JA, Dondon J, Nakamura Y, Grunberg-Manago M, « Effect of NusA protein on expression of the nusA,infB operon in E. coli », Nucleic Acids Res, vol. 13, no 9, , p. 3371-3388
- Nakamura Y, Plumbridge J, Dondon J, Grunberg-Manago M, « Evidence for autoregulation of the nusA-infB operon of Escherichia coli », Gene, vol. 36, nos 1-2, , p. 189-193
- Worbs M, Bourenkov GP, Bartunik HD, Huber R, Wahl MC, « An extended RNA binding surface through arrayed S1 and KH domains in transcription factor NusA », Mol Cell, vol. 7, no 6, , p. 1177-1189
Gammaproteo_rimP-leader alignment [1]