Discussion:Puce d'hybridation génomique comparative

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Technical considerations[modifier le code]

There are several requirements that are dependent on the application of aCGH:
*Complexity.  Measurement becomes difficult in larger organisms because of decreasing partial concentrations of each portion of the sequence that is involved in the
hybridization to the array element as the size of the genomes increase.  This issue may be addressed by increasing the threshold in which one detects only larger 
increases in copy number of DNA extracted from cells, but this comes at the cost of increasing failure to detect low level gains and losses.
*Samples.  Tissue specimens may contain heterogeneous cell populations, which may further decrease the ability to detect copy number change in genes in the 
aberrant tumor cells because the population may contain normal cells. Furthermore, the use of tissue from clinical specimens severely limit the amount of 
DNA available for analysis.
*Error tolerance.  If the investigator is set to obtain a generalized description of aberrations that may occur in a set of samples, then errors in the 
detection may not be critical.  However, the margin for error is drastically narrowed in a clinical setting, where an individual specimen is used to 
obtain specific information.
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Gbdivers (d) 21 novembre 2008 à 20:34 (CET)